New Delhi metallo-β-lactamase-1: detection and prevention.

1240 CMAJ, August 9, 2011, 183(11) © 2011 Canadian Medical Association or its licensors The advent of New Delhi metallo-β lactamase-1 (NDM-1) as a new mechanism of microbial resistance went mostly unmarked, despite it being plasmidmediated, highly transferable and able to confer resistance to most antibiotics (with the exceptions of colistin and tigecycline). However, after the Indian subcontinent was linked to the spread of organisms producing NDM-1, and in response to the resulting reaction from India’s Government, NDM-1 became an overnight global phenomenon. Thus far, most cases of infection or colonization with NDM-1– producing bacteria have originated in southern Asia, most often in India. It is now clear that NDM-1 is firmly established in India, both in the hospitals and in the community. At the community level, the situation is exacerbated by the poor sanitary conditions with which many Indians must live on a daily basis. From a diagnostic and microbiologic perspective, NDM-1 is not easily identified in clinical bacteria. Some methods of detection lack the sensitivity necessary for the rapid and assured detection of NDM-1 that facilitates antibiotic stewardship and the introduction of measures to control the spread of infection. The story of NDM-1 is still in its infancy; however, it has already eclipsed the Klebsiella pneumoniae carbapenemases in terms of the number of countries in which it is found. In addition, because of the promiscuous nature of the plasmids containing the NDM-1 gene, the enzyme has been found in many more species than have the K. pneumoniae carbapenemases. Furthermore, our knowledge of NDM-1 is compromised by poor case studies; additional information, such as carriage and persistence, is required. In their article for the CMAJ, Kus and colleagues present two important but contrasting case studies. The first patient, an 86-year-old man, had no contact with India or southern Asia. The Morganella morganii isolated from a culture of the patient’s urine showed sensitivity to meropenem based on results from the Vitek II automated susceptibility testing system (bioMérieux, France), but carbapenemases were not detected by the Modified Hodge Test. However, despite these results, the organism was NDM-1 positive. These data throw into considerable doubt the validity of some of the methods heavily relied upon by diagnostic laboratories for the detection of antibiotic resistance. That the patient had no primary contact with southern Asia (indeed, he had not travelled outside of Canada in the previous 10 years) may be touted as evidence by the people who wish to propagate the myth that NDM-1 is everywhere. However, the link between southern Asia and most NDM-1–producing isolates is overwhelming, and the spread of such bacteria to the international community, such that direct links to southern Asia cannot be confirmed, is inevitable. Another important feature of this particular case is that the isolate could be found in the patient’s urine five months after the original diagnosis, an unusual show of persistence. Clearly, the isolate is not overtly pathogenic; however, it is able to persist in unusual environments, indicating a possible link between antibiotic resistance and persistence rather than pathogenicity. Unfortunately, samples of the patient’s stool were not taken, which may have shown the presence of the M. morganii strain or other isolates that could have conferred the NDM-1 gene to the patient’s natural flora. In the second case, an NDM-1–producing isolate of Providencia rettgeri caused a urinary tract infection in a 71-year-old woman who had recently travelled to India. For this patient, the link to southern Asia is indisputable. The patient New Delhi metallo-β-lactamase-1: detection and prevention